基于网络药理学及动物实验探讨天蝎袪痛颗粒治疗偏头痛的作用机制*

作者：孙亚丽1，赵继业2,3，贺宇田2，秦瑛烁2，任国华2,3

单位：1.内蒙古医科大学，内蒙古 呼和浩特 010020； 2.内蒙古自治区中医医院，内蒙古 呼和浩特 010051； 3.省部共建中医湿证国家重点实验室内蒙古工作站，内蒙古 呼和浩特 010051

引用：引用：孙亚丽，赵继业，贺宇田，秦瑛烁，任国华.基于网络药理学及动物实验探讨天蝎袪痛颗粒治疗偏头痛的作用机制[J].中医药导报,2026,32(1):29-36.

DOI：10.13862/j.cn43-1446/r.2026.01.005

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摘要：

目的：通过网络药理学方法探讨天蝎袪痛颗粒治疗偏头痛的作用机制，并进行动物实验验证。方法：借助液相色谱-质谱法（LC-MS）检测天蝎祛痛颗粒的成分，结合TCMSP、HERB数据库分析其活性成分及其潜在作用靶点，通过GeneCards数据库获取偏头痛相关靶点，筛选出药物-疾病共同靶点；采用String平台进行PPI网络分析共同靶点间的相互作用并获得关键靶点；结合R语言对关键靶点进行基因本体论（GO）富集分析和京都基因与基因组百科全书（KEGG）通路富集分析，构建“中药-靶点-通路”网络图，预测天蝎祛痛颗粒治疗偏头痛的关键通路。通过建立硝酸甘油诱导的偏头痛大鼠模型，采用蛋白质印迹法（Western blotting，WB）检测蛋白激酶A（PKA）、反应结合蛋白（CREB）关键蛋白表达水平。结果：LC-MS检测出天蝎袪痛颗粒中49个关键活性成分，网络药理学筛选出治疗偏头痛的361个潜在作用靶点，PPI分析发现肿瘤坏死因子（TNF）、非受体酪氨酸激酶（SRC）、表皮生长因子受体（EGFR）、胰岛素（INS）为关键靶点；GO和KEGG分析发现这些靶点显著富集于cAMP信号通路；WB检测结果显示，天蝎袪痛颗粒低、中、高剂量组能均显著抑制PKA和CREB蛋白表达（P＜0.05），其中天蝎祛痛颗粒中剂量组效果最为明显。结论：天蝎袪痛颗粒治疗偏头痛具有多成分、多靶点的作用特点，可能与cAMP-PKA-CREB信号通路的调节相关。

关键词：偏头痛；天蝎袪痛颗粒；网络药理学；cAMP通路；大鼠

Abstract：

Objective: To explore the mechanism of Tianxie Qutong granules in treating migraine based on network pharmacology and verify it through animal experiments. Methods: The components of Tianxie Qutong granules were detected using liquid chromatography-mass spectrometry (LC-MS). Their active components and potential targets were analyzed with the TCMSP and HERB databases. Migraine-related targets were retrieved from the GeneCards database to identify the common drug-disease targets. PPI network analysis of the common targets was performed using the String platform to investigate their interactions and identify key targets. Gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment analyses of the key targets were performed using R language to construct a "herb-target-pathway" network and predict the key pathways involved in the treatment of migraine with Tianxie Qutong granules. A rat model of migraine was established by nitroglycerin induction. The expression levels of key proteins, including protein kinase A (PKA) and cAMP response element-binding protein (CREB), were detected by Western blotting (WB). Results: LC-MS identified 49 key active components in Tianxie Qutong granules. Network pharmacology screening revealed 361 potential targets for migraine treatment. PPI analysis identified tumor necrosis factor (TNF), nonreceptor tyrosine kinase (SRC), epidermal growth factor receptor (EGFR), and insulin (INS) as key targets. GO and KEGG analyses indicated that these targets were significantly enriched in the cAMP signaling pathway. WB results showed that low-dose, medium-dose and dose high-dose groups of Tianxie Qutong granules significantly inhibited the protein expression of PKA and CREB (P<0.05), with the medium-dose group of Tianxie Qutong granules showing the most pronounced effect. Conclusion: Tianxie Qutong granules exert their therapeutic effects on migraine through multiple components and targets, which may be related to the regulation of the cAMP-PKA-CREB signaling pathway.

Key words：migraine; Tianxie Qutong granules; network pharmacology; cAMP pathway; rat

发布时间：2026-01-30

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