“返魂草-法半夏”配伍治疗寒饮蕴肺型慢性支气管炎大鼠的药效作用研究*
作者：王 利1,2，李辰辉1，龚志悦1，黄玉婷1，李 娟1,3,4
单位：1.湖南中医药大学药学院，湖南 长沙 410208； 2.长沙医学院，湖南 长沙 410219； 3.湖南省中药活性物质筛选工程技术研究中心，湖南 长沙 410208； 4.湖南省中美老年性退行性疾病治疗药物国际联合研究中心，湖南 长沙 410208
引用：引用：王利，李辰辉，龚志悦，黄玉婷，李娟.“返魂草-法半夏”配伍治疗寒饮蕴肺型慢性支气管炎大鼠的药效作用研究[J].中医药导报,2025,31(9):50-54.
DOI：10.13862/j.cn43-1446/r.2025.09.008
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摘要：
目的：探讨返魂草-法半夏（AP）配伍治疗寒饮蕴肺型慢性支气管炎（CB）大鼠的药效作用。方法：采用烟熏法诱导建立SD大鼠寒饮蕴肺型CB模型。根据随机分组方法，将大鼠分为空白对照组、模型组、阳性对照组（肺宁颗粒：2.70 g/kg）及高、中、低剂量组（5.40 g、2.70 g、1.35 g/kg）。测量并记录大鼠体质量、体温、进食量、饮水量和脏器系数（肺）变化。采用酚红法检测祛痰效果。采用酶联免疫法检测肿瘤坏死因子-α（TNF-α）、转化生长因子-β1（TGF-β1）、白细胞介素-2（IL-2）、白细胞介素-4（IL-4）、丙二醛（MDA）和超氧化物歧化酶（SOD）的表达水平。HE染色检测肺组织形态变化。结果：与空白对照组相比，模型组大鼠体质量、进食量、饮水量、酚红排泄量，IL-2和SOD水平显著降低（P＜0.05），脏器系数（肺）、TNF-α、TGF-β、IL-4和MDA水平显著升高（P＜0.05）。与模型组相比，高剂量组显著增加了大鼠体质量、进食量、饮水量、酚红排泄量、IL-2和SOD水平（P＜0.05），显著降低了大鼠脏器系数（肺）及TNF-α、TGF-β、IL-4和MDA水平（P＜0.05）。高剂量组大鼠体质量、进食量、饮水量、酚红排泄量、脏器系数（肺）、IL-4、IL-2、SOD和MDA水平与阳性对照组比较，差异无统计学意义（P＞0.05）。高剂量组大鼠TNF-α和TGF-β水平显著低于阳性对照组（P＜0.05）。结论：高剂量AP可通过减少TNF-α、TGF-β、IL-4、MDA以及升高IL-2和SOD水平，降低肺部炎症，提高抗氧化应激能力，发挥治疗寒饮蕴肺型CB的作用。此外，高剂量AP配伍对寒饮蕴肺型CB大鼠TNF-α和TGF-β水平的调控优于肺宁颗粒。
关键词：慢性支气管炎；返魂草；法半夏；配伍；寒饮蕴肺型；炎症；大鼠
Abstract：
Objective: To explore the pharmacological
effects of compatibility of Fanhuncao (aleutian ragwort)-Fabanxia (pinelliae
rhizoma praeparatum) (AP) in the
treatment of chronic bronchitis (CB) with syndrome of cold fluid retention in
rats. Methods: The CB model with syndrome of cold fluid retention in SD rats
was established by smoke inhalation. The rats were randomly divided into normal
control group, model group, positive control group (Lung Ning granules, 2.70
g/kg), and high, medium, and low dose groups of AP (5.40, 2.70 and 1.35 g/kg).
The changes in body weight, body temperature, food intake, water intake, and
organ coefficient (lung) of the rats were observed and recorded. The
expectorant was detected by the phenol red method. The expression levels of
tumor necrosis factor-α (TNF-α), transforming growth factor-β (TGF-β),
interleukin-2 (IL-2), interleukin-4 (IL-4), malondialdehyde (MDA) and
superoxide dismutase (SOD) were detected by enzyme-linked immunosorbent assay
(ELISA). The morphological changes of lung tissue were detected by hematoxylin-eos
(HE) staining. Results: Compared with the normal control group, the body
weight, food intake, water intake, phenol red excretion, and the levels of IL-2
and SOD significantly decreased in model group (P＜0.05), while the
organ coefficient (lung) and the levels of TNF-α, TGF-β, IL-4 and
MDA significantly increased (P＜0.05). Compared with the model group, the high-dose group of AP
significantly increased the body weight, food intake, water intake, phenol red
excretion, and the levels of IL-2 and SOD of the rats (P＜0.05), while
decreased the organ coefficient (lung), the levels of TNF-α, TGF-β, IL-4, and
MDA (P＜0.05). Compared with the positive control group, there was no
significant difference in the body weight, food intake, water intake, phenol
red excretion, organ coefficient (lung), IL-4, IL-2, SOD, and MDA levels of the
rats the high-dose group of AP (P＞0.05), while the high-dose group of AP showed lower levels of TNF-α and TGF-β than
positive control group (P＜0.05). Conclusion: The high-dose AP may play a role in the treatment
of CB with syndrome of cold fluid retention in rats by decreasing levels of
TNF-α, TGF-β, IL-4, MDA, increasing IL-2 and SOD, reducing pulmonary
inflammation and enhancing antioxidant capacity. Moreover, the effect of
high-dose AP in controlling the levels of TNF-α and TGF-β in CB with syndrome
of cold fluid retention rats was superior to that of Lung Ning granules.
Key words：chronic bronchitis; Fanhuncao (aleutian ragwort); Fabanxia (pinelliae rhizoma praeparatum); compatibility; cold fluid retention in lung; inflammation; rat
发布时间：2026-01-08
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