雪莲口服液对胶原诱导关节炎大鼠Wnt信号通路成骨细胞相关因子表达的影响*

作者：张晨曦，王灵锐，丛 珊，罗 剑，罗 莉

单位：新疆医科大学第一附属医院，新疆 乌鲁木齐 830000

引用：引用：张晨曦，王灵锐，丛珊，罗剑，罗莉.雪莲口服液对胶原诱导关节炎大鼠Wnt信号通路成骨细胞相关因子表达的影响[J].中医药导报,2025,31(8):53-57.

DOI：10.13862/j.cn43-1446/r.2025.08.009

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摘要：

目的：观察雪莲口服液对胶原诱导关节炎（CIA）大鼠Wnt/β-catenin（β-连环蛋白）信号通路成骨细胞相关因子表达的影响，探讨雪莲口服液对缓解CIA大鼠骨破坏的可能作用机制。方法：将40只SD大鼠随机分为正常组、模型组、阳性药组和雪莲口服液组，每组10只，除正常组外余各组建立CIA大鼠模型。成模后药物治疗28 d，用关节炎指数评分法（AI）评估关节炎，苏木精-伊红（HE）染色观察大鼠关节软骨病理改变，Mankin评分（曼金评分）评估软骨损伤程度；酶联免疫吸附试验（ELISA）法检测血清中炎症因子肿瘤坏死因子-α（TNF-α）、白介素-1β（IL-1β）、IL-6表达水平；免疫组织化学染色法检测关节软骨中β-catenin、Runx2（Runt相关转录因子2）、Tafazzin（TAZ）和Osteocalcin（OCN）蛋白表达水平。结果：给药第2、3、4周时，雪莲口服液组大鼠的AI评分均显著低于模型组，时间因素与分组因素对AI评分的影响存在交互效应，差异有统计学意义（P<0.05）。雪莲口服液组大鼠的关节软骨病理形态改善，软骨细胞紊乱和潮线破坏减少。雪莲口服液组大鼠Mankin评分低于模型组（P<0.05）。雪莲口服液组大鼠血清中TNF-α、IL-1β和IL-6水平均低于模型组（P<0.05），其抗炎效果与阳性药组比较，差异无统计学差异（P>0.05）。雪莲口服液组大鼠关节软骨中成骨因子β-catenin、Runx2、TAZ及OCN蛋白表达均高于模型组（P<0.05）。结论：雪莲口服液能够改善CIA大鼠骨破坏和关节炎症状，可能与调节Wnt/β-catenin信号通路成骨因子Runx2、TAZ和OCN蛋白表达有关。

关键词：类风湿关节炎；雪莲口服液；Wnt/β-catenin信号通路；成骨因子；胶原诱导关节炎；大鼠

Abstract：

Objective: To observe the effect of Saussurea involucrata oral liquid on expression of Wnt/β-catenin signal pathway osteoblast-related factors in rats with collagen-induced arthritis (CIA), and to explore the possible mechanism of Saussurea involucrata oral liquid in alleviating bone destruction in CIA rats. Methods: Totally 40 SD rats were randomly divided into normal group, model group, positive drug group and Saussurea involucrata oral liquid group, 10 rats in each group. CIA rat models were established in all groups except normal group. After 28 days of drug intervention, the arthritis index score (AI score) was used to evaluate the arthritis index, and the pathological changes of articular cartilage were observed by HE staining. The degree of cartilage injury was evaluated by Mankin score, and the levels of tumor necrosis factor-α(TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) in serum were detected by enzyme-linked immunosorbent assay (ELISA) method. The expression of β-catenin, Runx2, Tafazzin (TAZ) and Osteocalcin (OCN) in articular cartilage were detected by immunohistochemistry. Results: At weeks 2, 3, and 4 of administration, the Saussurea involucrata oral liquid group showed lower AI scores than model group (P<0.05). A statistically significant interaction effect between time and grouping factors on AI scores was observed (P<0.05). Saussurea treatment improved articular cartilage morphology, reduced chondrocyte disorganization, and mitigated tidemark disruption. The Saussurea involucrata oral liquid group showed lower Mankin score than model group (P<0.05). Saussurea involucrata oral liquid group showed lower serum TNF-α, IL-1β and IL-6 than model group (P<0.05). There was no statistically significant difference in its anti-inflammatory effect compared to positive drug group (P>0.05). Saussurea involucrata oral liquid group showed higher expression of β-catenin, Runx2, TAZ, and OCN than model group (P<0.05). Conclusion: Saussurea involucrata oral liquid can ameliorate the symptoms of bone destruction and arthritis in CIA rats, which may be related to regulating the expression of Runx2, TAZ and OCN of Wnt/β-catenin signal pathway.

Key words：rheumatoid arthritis; Saussurea involucrata oral liquid; Wntβ-catenin signaling pathway; osteogenic factor; collagen-induced arthritis; rat

发布时间：2026-01-06

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