基于突触可塑性探讨养脑柔筋方对脑梗死后痉挛性瘫痪大鼠运动功能障碍的影响*
作者：孙雄星1,2，曾珊珊2，吴玲应1,2，林仕高1,2，胡腾辉1,2，王释亮2，伍大华2，谢 乐2
单位：1.湖南中医药大学研究生院，湖南 长沙 410208； 2.湖南省中西医结合医院/湖南省中医药研究院附属医院，湖南 长沙 410006
引用：引用：孙雄星，曾珊珊，吴玲应，林仕高，胡腾辉，王释亮，伍大华，谢乐.基于突触可塑性探讨养脑柔筋方对脑梗死后痉挛性瘫痪大鼠运动功能障碍的影响[J].中医药导报,2025,31(8):17-23.
DOI：10.13862/j.cn43-1446/r.2025.08.004
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摘要：
目的：探讨养脑柔筋方对脑梗死后痉挛性瘫痪大鼠梗死灶周围突触可塑性相关蛋白表达的影响。方法：将雄性SD大鼠随机分为假手术组、模型组、阳性药组及中药组。采用线栓改良法建立大鼠永久性脑梗死模型，通过多通道电生理仪对各组大鼠进行H反射检测，第7、14、21、28天对各组大鼠进行改良神经功能缺损评分（mNSS）、转棒疲劳实验。末次给药后，采集脑组织样本进行HE染色及免疫荧光染色后分析评估大鼠梗死周围区域与突触可塑性相关的突触素（SYN）、突触后致密蛋白95（PSD-95）和突触后支架蛋白1（Homer1）的表达水平。结果：与假手术组比较，模型组大鼠运动能力下降，mNSS评分升高，Hmax/Mmax比值增加，SYN、PSD-95、Homer1蛋白表达显著下降（P<0.01或P<0.05）。与模型组比较，中药组大鼠运动能力显著增加（P<0.01）、mNSS评分显著降低（P<0.01）、Hmax/Mmax比值显著下降（P<0.05），且SYN、PSD-95、Homer1蛋白表达均明显增加（P<0.001）。结论：养脑柔筋方可能通过调控脑梗死后痉挛性瘫痪突触可塑性相关蛋白SYN、PSD-95、Homer1，从而改善大鼠的运动功能障碍。
关键词：脑梗死后痉挛性瘫痪；突触可塑性；养脑柔筋方；SYN；PSD-95；Homer1；大鼠
Abstract：
Objective: To investigate the effects of
Yangnao Roujin formula (YNRJF) on the expression of synaptic plasticity-related
proteins in the peri-infarct region of rats with post-stroke spasticity (PSS).
Methods: Male Sprague-Dawley (SD) rats were randomly divided into sham surgery
group, model group, positive control group (baclofen) and YNRJF group. A
permanent cerebral infarction model was established using the modified
thread-occlusion method. H-reflex testing was performed using a multi-channel
electrophysiological system. Modified Neurological Severity Score (mNSS) and
rotarod fatigue test were assessed on days 7, 14, 21, and 28. After the final
administration, brain tissues were collected for hematoxylin-eosin (HE)
staining and immunofluorescence to analyze the expression levels of synaptic
plasticity-related proteins [synapsin (SYN), postsynaptic density-95 (PSD-95)
and Homer1] in the peri-infarct region. Results: Compared with the sham group,
the model group exhibited reduced motor function, increased mNSS scores,
elevated Hmax/Mmax ratio, and decreased expression of SYN, PSD-95, and
Homer1(P<0.01 or P<0.05). Compared with the model group, the YNRJF group
showed improved motor function (P<0.01), reduced mNSS scores (P<0.01),
decreased Hmax/Mmax ratio (P<0.05), and markedly increased expression of
SYN, PSD-95 and Homer1 (P<0.001). Conclusion: YNRJF may ameliorate motor
dysfunction in PSS rats by regulating synaptic plasticity-related proteins
(SYN, PSD-95 and Homer1).
Key words：post-stroke spasticity ; synaptic plasticity;Yangnao Roujin formula; SYN; PSD-95; Homer1; rat
发布时间：2026-01-06
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