摩腹对功能性便秘大鼠肠道菌群的影响*

作者：何丹丹1，张 贤1，刘小峰2，邵家豪2，廖翼涛2，陈 莉1，杭晓娟1，吴昔钧1

单位：1.南京中医药大学附属无锡医院，江苏 无锡 214071； 2.南京中医药大学，江苏 南京 210023

引用：引用：何丹丹，张贤，刘小峰，邵家豪，廖翼涛，陈莉，杭晓娟，吴昔钧.摩腹对功能性便秘大鼠肠道菌群的影响[J].中医药导报,2025,31(6):15-20.

DOI：10.13862/j.cn43-1446/r.2025.06.003

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摘要：目的：探讨摩腹对盐酸洛哌丁胺诱导的功能性便秘大鼠的治疗作用及肠道菌群的影响。方法：将40只SD大鼠随机分为空白（normal，N）组、模型（model，M）组、乳果糖（lactulos，L）组和推拿（tuina，TN）组，每组10只。第1~14天，M组、L组和TN组采用盐酸洛哌丁胺胶囊粉末制成的混悬液灌胃（3 mg/kg）建立功能性便秘大鼠模型，N组采用等体积生理盐水灌胃。第15~28天，N组和M组给予生理盐水灌胃（10 mL/kg），L组给予乳果糖灌胃（2.57 mL/kg），TN组给予摩腹干预，均1次/d。治疗结束后，检测各组大鼠粪便含水率与肠推进率，将收集的大鼠新鲜粪便进行16SrDNA检测，分析样本肠道菌群的变化。结果：与N组比较，M组大鼠粪便含水率明显下降（P＜0.05），肠推进率明显降低（P＜0.05），肠道菌群紊乱，Firmicuts、Oscillospiraceae丰度下降，而Bacteroidetes、Muribaculaceae、Prevotellaceae丰度增加；与M组比较，L组和TN组粪便含水量明显上升（P＜0.05），肠推动率明显升高（P＜0.05），肠道菌群多样性增加（P＜0.01），Bacteroidetes、Muribaculacea、Prevotellaceae丰度下降，而Firmicuts、Lachnospiraceae、Oscillospiraceae丰度增加。LEfSe分析表明，TN组c-clostridia、p-Firmicutes、o-Lachnospirales、f-Lachnospiraceae和o-Oscillospirales等为丰度较高的差异物种。结论：摩腹可通过调整大鼠肠道菌群结构改善大鼠便秘状态。

关键词：功能性便秘；摩腹；肠道菌群；16SrDNA；大鼠

Abstract：

Objective: To investigate the therapeutic effect and intestinal microbiota of abdominal friction on fluperamide-induced functional constipation in rats. Methods: A total of 40 SD rats were randomly divided into normal group (group N), model group (group M), lactulose group (group L) and Tuina group (group TN), with 10 rats in each group. On the lst to 14th days, group M, group L and group TN were gavaged with a suspension of loperamide hydrochloride capsule powder (3 mg/kg), and group N was gavaged with the same volume of normal saline. On the 15th to 28th days, group N and group M were given normal saline (10 mL/kg), and group L was given lactulose (2.57 ml/kg), once a day. Group TN was given abdominal friction intervention, once a day. After the end of treatment, the fecal moisture content and intestinal propulsion rate of rats in each group were detected, and the collected fresh feces of rats were tested for 16SrDNA to analyze the changes of intestinal flora in the samples. Results: Compared with the group N, the fecal water content decreased significantly in group M (P＜0.05), and the intestinal propulsion rate decreased significantly in group M (P＜0.05). There was disruption of gut microbiota in the group M. The abundance of Firmicuts and Lactobacillaceae decreased in group M, while the abundance of Bacteroidetes, Muribaculaceae, Lachnospiraceae and Prevotellaceae increased in group M. Compared with the group M, the fecal water content increased significantly in group L and group TN (P＜0.05), and the intestinal propulsion rate increased significantly in group L and group TN (P＜0.05). The abundances of Bacteroidetes, Muribaculacea and Prevotellaceae decreased in group L and group TN, while the abundances of Firmicuts, Lachnospiraceae and Oscillospiraceae increased group L and group TN. LEfSe analysis showed that c-clostridia, p-firmicutes, o-Lachnospirales, f-Lachnospiraceae and o-Oscillospirales were the most abundant differential species in the TN group. Conclusion: By adjusting the structure of the intestinal flora of rats, abdominal friction can improve the constipation state of rats.

Key words：functional constipation; abdominal friction; intestinal flora; 16SrDNA; rat

发布时间：2026-01-03

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