二十味肉豆蔻丸改善戊四唑致小鼠焦虑样行为和HPG轴的作用研究*

作者：鲍晓虹，叶田园，程肖蕊

单位：山东中医药大学中医药创新研究院，山东 济南 250355

引用：引用：鲍晓虹，叶田园，程肖蕊.二十味肉豆蔻丸改善戊四唑致小鼠焦虑样行为和HPG轴的作用研究[J].中医药导报,2026,32(1):92-97.

DOI：10.13862/j.cn43-1446/r.2026.01.015

PDF： 下载PDF

摘要：

目的：研究藏药二十味肉豆蔻丸对单次腹腔注射戊四唑（PTZ）诱导ICR小鼠焦虑样行为的影响，并从下丘脑-垂体-性腺（HPG）轴角度初步探讨其作用机制。方法：单次腹腔注射PTZ后，灌胃给予ICR小鼠二十味肉豆蔻丸，采用O迷宫、高架十字迷宫、明暗箱、旷场实验检测其对PTZ诱导的焦虑样行为的影响，采用ELISA方法检测小鼠下丘脑促性腺激素释放激素（GnRH）、垂体促卵泡成熟素（FSH）、黄体生成素（LH）、血浆雄激素（T）和雌激素（E2）的含量。结果：单次腹腔注射PTZ能够引起小鼠焦虑样行为，二十味肉豆蔻丸可显著延长小鼠明暗箱实验中明箱停留时间、提高明箱停留时间百分比；在旷场实验中可显著延长小鼠运动总距离、增加进入中央区的次数、增加中央区运动距离、提高中央区运动距离百分比。二十味肉豆蔻丸可促进小鼠垂体中LH的分泌、增加血浆中T的含量。结论：二十味肉豆蔻丸可改善单次腹腔注射PTZ诱导的ICR小鼠焦虑样行为，其机制可能在于增强HPG轴功能，调节γ氨基丁酸（GABA）能神经传递。

关键词：焦虑；二十味肉豆蔻丸；下丘脑-垂体-性腺轴；戊四唑；小鼠

Abstract：

Objective: To study the effect of the Zang medicine Ershiwei Roudoukou Wan on anxiety-like behavior induced by a single intraperitoneal injection of pentylenetetrazole (PTZ) in ICR mice and to preliminarily explore its mechanism from the perspective of the hypothalamic-pituitary-gonadal (HPG) axis. Methods: After modeling via a single intraperitoneal injection of PTZ, ICR mice were administered Ershiwei Roudoukou Wan by gavage. The effects on PTZ-induced anxiety-like behavior were assessed using the O maze, elevated plus maze, light-dark box, and open field test. The levels of gonadotropin-releasing hormone (GnRH) in the hypothalamus, as well as follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in the pituitary, and plasma testosterone (T) and estradiol (E2) were measured by enzyme-linked immunoadsordent assay  (ELISA). Results: A single intraperitoneal injection of PTZ induced anxiety-like behavior in mice. Treatment with Ershiwei Roudoukou Wan significantly increased the time spent in the light compartment and the percentage of time in the light compartment in the light-dark box test. In the open field test, it significantly increased the total movement distance, the number of entries into the central area, the movement distance within the central area, and the percentage of central area movement distance. Furthermore, Ershiwei Roudoukou Wan promoted the secretion of LH in the pituitary and increased the plasma T level. Conclusion: Ershiwei Roudoukou Wan can improve anxiety-like behavior in ICR mice induced by a single intraperitoneal injection of PTZ. The mechanism may involve enhancing the function of the HPG axis and regulating gamma-aminobutyric acid (GABA)-ergic neurotransmission.

Key words：anxiety; Ershiwei Roudoukou Wan; HPG axis; pentylenetetrazole; mice

发布时间：2026-01-30

点击量：0