基于一测多评法同时测定菟芪合剂中6种关键成分*
作者：杨扬宇1,2，谢 谦1,2，邱盼子1,2，肖作奇1,2，欧阳波1,2
单位：1.湖南省妇幼保健院，湖南 长沙 410008； 2.妇儿中医临床验方转化湖南省工程研究中心，湖南 长沙 410008
引用：引用：杨扬宇，谢谦，邱盼子，肖作奇，欧阳波.基于一测多评法同时测定菟芪合剂中6种关键成分[J].中医药导报,2026,32(1):87-91.
DOI：10.13862/j.cn43-1446/r.2026.01.014
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摘要：
目的：建立能同时测定菟芪合剂中芍药苷、松脂醇二葡萄糖苷、毛蕊异黄酮葡萄糖苷、甘草苷、金丝桃苷、川续断皂苷Ⅵ含量的一测多评定量分析方法。方法：采用高效液相色谱法，利用Thermo Hypersil C18色谱柱（250.0 mm×4.6 mm，5.0 μm）；流动相为0.1%磷酸溶液-甲醇，梯度洗脱；流速为1.0 mL/min；柱温为30 ℃；DAD检测器。以甘草苷为内参物，分别构建其余5种成分的相对校正因子，进而计算含量。结果：芍药苷、松脂醇二葡萄糖苷、毛蕊异黄酮葡萄糖苷、金丝桃苷、川续断皂苷Ⅵ的相对校正因子分别为2.037 0、0.407 9、0.512 7、0.827 8、9.656 1；12批菟芪合剂中6个成分的一测多评法所得结果与外标法的测定值无显著差别。结论：该方法高效快速，结果准确、可靠，可用于菟芪合剂中6种成分的同时测定，为菟芪合剂的质量控制及标准建立提供了依据。
关键词：菟芪合剂；一测多评；含量测定；高效液相色谱
Abstract：
Objective: To establish a method for the simultaneous determination of paeoniflorin, pinoresinol diglucoside, calycosin-7-O-β-D-glucoside, liquiritin, hyperoside, and asperosaponin Ⅵ in Tuqi mixture using quantitative analysis of multi-components by single marker (QAMS). Methods: A high-performance liquid chromatography (HPLC) method was employed. The separation was performed on a Thermo Hypersil C18 column (250.0 mm×4.6 mm, 5.0 μm). The mobile phase consisted of methanol and 0.1% phosphoric acid solution with gradient elution at a flow rate of 1.0 mL/min. The column temperature was maintained at 30 °C, and detection was carried out using a DAD detector. With liquiritin as the internal reference substance, the relative correction factors (RCFs) of the other five components were calculated to determine their contents. Results: The RCFs for paeoniflorin, pinoresinol diglucoside, calycosin-7-O-β-D-glucoside, hyperoside, and asperosaponin Ⅵ were 2.037 0, 0.407 9, 0.512 7, 0.827 8, and 9.656 1, respectively. For 12 batches of Tuqi mixture, the results obtained by the QAMS method for the six components showed no significant difference compared with those determined by the external standard method. Conclusion: The established method is efficient, rapid, accurate, and reliable, and it can be used for the simultaneous determination of the six components in Tuqi mixture, providing a basis for the quality control and standard establishment of Tuqi mixture.
Key words：Tuqi mixture; QAMS; content determination; HPLC
发布时间：2026-01-30
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