心俞埋线联合培哚普利基于抑制CaMKⅡ活性调节心肌梗死大鼠炎症消退介质的合成*

作者：穆泉洁1，黄小楼1，邰 萱1，张 婷1，薛 覃1，刘雨桔1，吴高鑫1,2

单位：1.贵州中医药大学针灸推拿学院，贵州 贵阳 550025； 2.贵州中医药大学第一附属医院，贵州 贵阳 550001

引用：引用：穆泉洁，黄小楼，邰萱，张婷，薛覃，刘雨桔，吴高鑫.心俞埋线联合培哚普利基于抑制CaMKⅡ活性调节心肌梗死大鼠炎症消退介质的合成[J].中医药导报,2026,32(1):71-76.

DOI：10.13862/j.cn43-1446/r.2026.01.011

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摘要：

目的：探讨心俞穴位埋线联合培哚普利改善急性心肌梗死大鼠炎症的可能机制。方法：采用左冠状动脉前降支结扎法建立急性心肌梗死模型，将造模成功大鼠分为模型组、埋线组、培哚普利组、埋线联合培哚普利组（线药组），每组15只，另取15只大鼠作为假手术组，假手术组仅开胸、穿线，不结扎，术后第2天开始干预。培哚普利组予以0.4 mg/（kg·d）培哚普利溶液灌胃，埋线组给予心俞穴埋线，线药组给予心俞穴埋线和培哚普利组同剂量药物灌胃，假手术组和模型组仅予以等量生理盐水灌胃，并于治疗3 d、7 d、28 d后3个时间点观察。HE染色观察心肌组织病理形态；酶联免疫吸附试验（ELISA）检测大鼠血清中白三烯B4（LTB4）、脂氧素（LXA4）水平；蛋白质印迹（Western blotting）法检测心肌组织p-CaMKⅡ、Ca2+-钙调蛋白依赖性蛋白激酶Ⅱ（CaMKⅡ）蛋白表达。结果：与假手术组比较，治疗3 d、7 d、28 d后模型组大鼠可见心肌细胞肿胀、部分细胞核游离、排列无序及炎症细胞浸润，心肌细胞横截面积和血清LTB4水平、CaMKⅡ、p-CaMKⅡ蛋白表达增加，血清LXA4水平降低（P<0.05）；治疗3 d、7 d和28 d时，与模型组比较，除3 d埋线组，其他治疗组大鼠心肌细胞形态、排列及炎症细胞浸润程度均有不同程度的改善，心肌细胞横截面积和血清LTB4水平、CaMKⅡ、p-CaMKⅡ蛋白表达均有不同程度降低（P<0.05），血清LXA4水平均不同程度增加（P<0.05）；线药组疗效优于其他治疗组（P<0.05）。与治疗3 d后同治疗组比较，治疗7 d、28 d后各治疗组血清LTB4水平和CaMKⅡ、p-CaMKⅡ蛋白表达不同程度降低（P<0.05），LXA4水平均不同程度升高（P<0.05）；与治疗7 d各治疗组比较，治疗28 d各治疗组血清LTB4水平和CaMKⅡ、p-CaMKⅡ蛋白表达不同程度降低（P<0.05），LXA4水平均不同程度升高（P<0.05），28 d线药组疗效最佳（P<0.05）。结论：心俞穴埋线联合培哚普利可能具有协同增效的作用，可更好发挥对急性心肌梗死后心肌梗死的保护作用，其疗效呈时间依赖性增强趋势，作用机制可能与抑制CaMKⅡ蛋白活性，增加血清LXA4水平，降低血清LTB4水平，抑制心肌梗死后过度炎症，促进心肌损伤修复有关。

关键词：心肌梗死；穴位埋线；培哚普利；CaMKⅡ；炎症消退介质；大鼠

Abstract：Objective: To investigate the potential mechanism by which thread-embedding at the "Xinshu" (BL 15)  combined with perindopril improves inflammation in rats with acute myocardial infarction (AMI). Methods: The AMI model was established by ligating the left anterior descending coronary artery. Successfully modeled rats were divided into the model group, thread-embedding group, perindopril group, and thread-embedding combined with perindopril (combination) group, with 15 rats in each group. Another 15 rats were assigned to the sham-operated group, which underwent thoracotomy and threading without ligation. Interventions began on the second day post-operation. The perindopril group received perindopril solution at 0.4 mg/(kg·d) by gavage. The thread-embedding group received thread-embedding at the "Xinshu" (BL 15). The combination group received both thread-embedding at the "Xiushu" (BL 15) and perindopril gavage at the same dose. The sham-operated group and model group received an equal volume of saline. Observations were madeafter 3, 7, and 28 days of treatment. Pathological morphology of myocardial tissue was observed by hematoxylin-eosin (HE) staining. Serum levels of LTB4 and LXA4 were detected by enzyme-linked immunosorbent assay (ELISA). Protein expression of phosphorylated calmodulin-dependent protein kinase Ⅱ (p-CaMKⅡ) and calmodulin-dependent protein kinase Ⅱ(CaMKⅡ) in myocardial tissue was detected by Western blotting. Results: Compared with the sham-operated group, the model group showed cardiomyocyte swelling, partial nuclear lysis, disordered arrangement, inflammatory cell infiltration, increased cardiomyocyte cross-sectional area and serum LTB4 levels, increased CaMKⅡ and p-CaMKⅡ protein expression, and decreased serum LXA4 levels (P<0.05) after 3, 7, and 28 days of treatment. At 3, 7, and 28 days, compared with the model group, all treatment groups except the thread-embedding group at 3 days showed varying degrees of improvement in cardiomyocyte morphology, arrangement, and inflammatory cell infiltration. The cardiomyocyte cross-sectional area, serum LTB4 levels, and CaMKⅡ and p-CaMKII protein expression were decreased to varying degrees (P<0.05), while serum LXA4 levels were increased to varying degrees (P<0.05). The combination group showed superior efficacy compared to the other treatment groups (P<0.05). Within each treatment group, compared to the 3-day time point, the serum LTB4 levels and CaMKⅡ and p-CaMKⅡ protein expression at 7 and 28 days were significantly decreased (P<0.05), while LXA4 levels were increased (P<0.05). Furthermore, compared to the 7-day time point, the serum LTB4 levels and CaMKⅡ and p-CaMKⅡ protein expression at 28 days were significantly decreased (P<0.05), while LXA4 levels were increased (P<0.05), with the 28-day combination group showing the best efficacy (P<0.05). Conclusion: Thread-embedding at the "Xinshu" (BL15) combined with perindopril may have a synergistic effect, providing better myocardial protection after AMI in a time-dependent manner. The mechanism may be related to inhibiting CaMKⅡ protein activity, thereby increasing serum LXA4 levels, decreasing serum LTB4 levels, suppressing excessive post-infarction inflammation, and promoting myocardial repair.

Key words：myocardial infarction; acupoint thread-embedding; perindopril; CaMKⅡ; inflammatory resolution mediators; rat

发布时间：2026-01-30

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