定喘颗粒调控AMPK/PGC-1α信号通路干预幼龄大鼠呼吸道合胞病毒肺炎的机制*

作者：李兆洋1，张秀英2，赵航宇1，魏晨浩1，张 来1，王 蕊1

单位：1.辽宁中医药大学，辽宁 沈阳 110032； 2.辽宁中医药大学附属医院，辽宁 沈阳 110032

引用：引用：李兆洋，张秀英，赵航宇，魏晨浩，张来，王蕊.定喘颗粒调控AMPK/PGC-1α信号通路干预幼龄大鼠呼吸道合胞病毒肺炎的机制[J].中医药导报,2025,31(10):22-26.

DOI：10.13862/j.cn43-1446/r.2025.10.004

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摘要：

目的：探讨定喘颗粒通过调控腺苷酸活化蛋白激酶（AMPK）/过氧化物酶体增殖物激活受体γ共激活因子-1α（PGC-1α）信号通路干预幼龄大鼠呼吸道合胞病毒（RSV）肺炎的作用机制。方法：将60只幼龄SD大鼠随机分为正常组、模型组、阳性对照组、定喘颗粒低剂量组、定喘颗粒中剂量组及定喘颗粒高剂量组，每组10只。采用RSV滴鼻法复制RSV肺炎幼鼠模型。阳性对照组予利巴韦林[0.15 g/（kg·d）]灌胃，定喘颗粒低、中、高剂量组分别予0.55、1.10、2.20 g/（kg·d）定喘颗粒灌胃，均连续灌胃7 d后取材。HE染色法观察肺组织病理形态，共聚焦荧光定位法检测肺组织活性氧（ROS）水平，酶联免疫吸附试验（ELISA）检测肺泡灌洗液（BALF）中白介素-18（IL-18）、IL-1β含量，蛋白质印迹法（Western blotting）检测肺组织中AMPK、PGC-1α蛋白表达水平。结果：干预7 d后，与模型组比较，定喘颗粒治疗后能够改善RSV肺炎大鼠肺组织病理损伤（P<0.05），降低肺组织活性氧（ROS）水平（P<0.05），显著降低BALF中IL-1β、IL-18水平（P<0.05），显著升高AMPK、PGC-1α蛋白表达（P<0.05）。结论：定喘颗粒可能通过上调AMPK/PGC-1α信号通路，降低肺组织ROS水平，抑制IL-18、IL-1β炎症因子释放，减轻肺组织病理损伤，从而改善RSV幼龄大鼠肺部炎症。

关键词：呼吸道合胞病毒肺炎；定喘颗粒；腺苷酸活化蛋白激酶；过氧化物酶体增殖物激活受体γ共激活因子-1α；活性氧；大鼠

Abstract：

Objective: To explore the mechanism of Dingchuan granule in preventing respiratory syncytial virus (RSV) pneumonia in young rats by regulating AMPK/PGC-1α signaling pathway. Methods: Totally 60 young SD rats were randomly assigned to normal group, model group, positive control group, low-dose Dingchuan granule group, medium-dose Dingchuan granule group, and high-dose Dingchuan granule group, 10 rats in each group. RSV nasal drip method was used to replicate RSV pneumonia mouse model. The positive control group was given ribavirin [0.15 g/(kg·d)] by gavage. The low-dose Dingchuan granule group, medium-dose Dingchuan granule group, and high-dose Dingchuan granule group were given Dingchuan granules with doses of 0.55, 1.10, and 2.20 g/(kg·d), respectively. The samples were taken after 7 days of continuous gavage. HE staining method was used to observed the pathological morphology of lung tissue. Confocal fluorescence localization method was used to detect the reactive oxygen species (ROS) levels in lung tissue. Enzyme-linked immunoadsordent assay (ELISA) was used to detect the IL-18 and IL-1β in BALF. The protein expression levels of AMPK and PGC-1α in lung tissue were detected by Western blotting. Results: After 7 days of intervention, compared with the model group, Dingchuan granule could improve the pathological damage of lung tissue in the rats with RSV pneumonia (P<0.05), reduce the level of ROS in lung tissue (P<0.05), decrease the levels of IL-1β and IL-18 in BALF (P<0.05), and increase protein expressions of AMPK and PGC-1α (P<0.05). Conclusion: Dingchuan granule may reduce the level of ROS in lung tissue, inhibit the release of IL-18 and IL-1β by up-regulating AMPK/PGC-1α signaling pathway, alleviate pathological damage of lung tissue, and improve pulmonary inflammation in young RSV rats.

Key words：respiratory syncytial virus pneumonia; Dingchuan granule; AMPK; PGC-1α; ROS; rat

发布时间：2026-01-08

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