小儿久咳合剂对感染后咳嗽大鼠SUMF1/SUMF2平衡的调节作用及对IL-13分泌的影响*

作者：李霁航1，郭育辰1，朱宇子1，吴哲伟1，周 智2，刘靖雷1，罗银河1

单位：1.湖南中医药大学，湖南 长沙 410208； 2.长沙市妇幼保健院，湖南 长沙 410000

引用：引用：李霁航，郭育辰，朱宇子，吴哲伟，周智，刘靖雷，罗银河.小儿久咳合剂对感染后咳嗽大鼠SUMF1/SUMF2平衡的调节作用及对IL-13分泌的影响[J].中医药导报,2025,31(9):38-43,49.

DOI：10.13862/j.cn43-1446/r.2025.09.006

PDF： 下载PDF

摘要：目的：研究小儿久咳合剂调节感染后咳嗽大鼠SUMF1/SUMF2平衡的效果及其对IL-13分泌水平的调控作用。方法：将40只SD大鼠随机分为空白组（10只）、模型组（10只）、中药组（10只）及西药组（10只）。通过烟熏暴露、LPS鼻腔滴注联合辣椒素雾化刺激，构建大鼠感染后咳嗽（PIC）模型。中药组灌服1.80 g/mL小儿久咳合剂溶液，西药组予0.08 mg/mL孟鲁司特纳水溶液灌胃，空白组和模型组给予等体积0.9%氯化钠溶液灌胃。通过Masson、PAS及HE染色观察肺组织病理变化；ELISA法检测血清及支气管肺泡灌洗液（BALF）中总IgE、特异性IgE及IL-13水平；Western blotting及PCR技术检测肺组织中SUMF1、SUMF2及IL-13的表达。结果：病理切片显示，模型组大鼠出现显著的气管上皮鳞状化生、密集的炎症细胞浸润和肺间质扩张，与空白组形成鲜明对比。肺组织中SUMF1、SUMF1/SUMF2比值及IL-13表达显著升高（P<0.01），而SUMF2表达显著降低（P<0.01），中性粒细胞和巨噬细胞密度显著升高（P＜0.01）。血清及BALF中总IgE、特异性IgE及IL-13表达均显著升高（P<0.01）。与模型组比较，中药组及西药组大鼠肺组织炎症细胞浸润及气管上皮损伤显著减轻，SUMF1、SUMF1/SUMF2比值及IL-13表达显著降低（P<0.01），而SUMF2表达显著升高（P<0.01），炎症细胞密度显著降低（P<0.01）。血清及BALF中总IgE、特异性IgE及IL-13表达亦显著降低（P<0.01）。结论：小儿久咳合剂可通过恢复SUMF1/SUMF2平衡、抑制IL-13的活性和分泌，减轻气道炎症，是其发挥治疗效应的可能机制。

关键词：感染后咳嗽；气道炎症；小儿久咳合剂；磷酸酯酶修饰因子；白细胞介素-13；大鼠

Abstract：Objective: To investigate the effect of Xiao'er Jiuke mixture on regulating the SUMF1/SUMF2 balance and its modulatory role on IL-13 secretion levels in rats with post-infectious cough (PIC). Methods: Totally 40 Sprague-Dawley (SD) rats were randomly assigned into blank control group (n=10), model group (n=10), Chinese medicine group (n=10), and Western medicine group (n=10). A PIC model was established using a combination of smoke exposure, lipopolysaccharide (LPS) nasal instillation, and capsaicin aerosol challenge. The Chinese medicine group was administered Xiao'er Jiuke mixture solution (1.80 g/mL) via oral gavage. The Western medicine group received Montelukast sodium solution (0.08 mg/mL) via oral gavage, and the blank control group and model group received an equal volume of 0.9% sodium chloride solution via oral gavage. Pathological changes in lung tissue were assessed using Masson, periodic acid-Schiff (PAS), and hematoxylin-eosin (HE) staining. Serum and bronchoalveolar lavage fluid (BALF) levels of total IgE, specific IgE, and IL-13 were measured by enzyme-linked immunosorbent assay (ELISA). The expression levels of SUMF1, SUMF2, and IL-13 in lung tissue were determined by Western blotting (WB) and polymerase chain reaction (PCR). Results: Compared to the blank control group, lung tissue sections from the model group exhibited significant tracheal epithelial squamous metaplasia, extensive inflammatory cell infiltration, and pulmonary interstitial expansion. Lung tissue levels of SUMF1, the SUMF1/SUMF2 ratio, and IL-13 were significantly increased in model group (P<0.01), while SUMF2 levels were significantly decreased (P<0.01). Neutrophil and macrophage densities were also significantly elevated in model group (P<0.01). Furthermore, serum and BALF levels of total IgE, specific IgE, and IL-13 were all significantly increased in model group (P<0.01) compared to the blank control. Compared to the model group, Chinese medicine group and Western medicine group showed significantly reduced inflammatory cell infiltration and tracheal epithelial damage in lung tissue. Levels of SUMF1, SUMF1/SUMF2 ratio, and IL-13 were significantly decreased in Chinese medicine group and Western medicine group (P<0.01), while SUMF2 levels were significantly increased (P<0.01). Inflammatory cell densities were also significantly reduced in Chinese medicine group and Western medicine group (P<0.01). Serum and BALF levels of total IgE, specific IgE, and IL-13 were likewise significantly decreased in Chinese medicine group and Western medicine group (P<0.01) compared to the model group. Conclusion: Xiao'er Jiuke Mixture may exert its therapeutic effect by restoring the balance of SUMF1/SUMF2, inhibiting the activity and secretion of IL-13, and reducing airway inflammation. 

Key words：coughing after infection; airway inflammation; Xiao'er Jiuke mixture; phosphatase modifying factor; interleukin-13; rat

发布时间：2026-01-08

点击量：38