非酒精性脂肪性肝病患者齿痕舌与肠道菌群的相关性分析*

作者：吴巧玲1，2，贺怡宁1，王 云1，李牛牛1，朱 磊3，赵 崧3

单位：1.南京中医药大学，江苏 南京 210000； 2.宁德市中医院，福建 宁德 352000； 3.南京中医药大学附属医院，江苏 南京 210000

引用：引用：吴巧玲，贺怡宁，王云，李牛牛，朱磊，赵崧.非酒精性脂肪性肝病患者齿痕舌与肠道菌群的相关性分析[J].中医药导报,2025,31(7):98-104,109.

DOI：10.13862/j.cn43-1446/r.2025.07.016

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摘要：目的：探讨非酒精性脂肪性肝病（NAFLD）伴齿痕舌患者的肠道菌群结构。方法：招募NAFLD患者73例，并分为齿痕舌组30例及非齿痕舌组43例，收集患者的粪便标本。利用Illumina Novaseq 6000测序仪对粪便总DNA中的16S rDNA V3-V4区域进行高通量测序，使用生物信息学软件进行肠道菌群结构分析。结果：齿痕舌组患者肠道菌群丰富度和多样性明显降低。齿痕舌组患者乳杆菌属丰度低于非齿痕舌组（P<0.05）。基于LEfSe和随机森林分析构建预测模型，AUC值为97.9%。基于京都基因与基因百科组全书（KEGG）通路富集差异分析，发现齿痕舌组患者肠道菌群中包含内分泌代谢、脂质代谢在内的代谢通路功能水平下降。结论：非酒精性脂肪性肝病伴有齿痕舌患者的肠道菌群紊乱更为显著，表现为肠道菌群丰富度显著降低，其中在属水平上以乳杆菌属丰度显著降低为特点。

关键词：非酒精性脂肪性肝病；齿痕舌；乳杆菌属；肠道菌群

Abstract：

Objective: To investigate the characteristics of gut microbiota in patients with non-alcoholic fatty liver disease (NAFLD) and tooth mark tongue. Methods: A total of 73 patients with NAFLD were recruited, and divided into two groups, 30 patients in the tooth mark tongue group (TMT) and 43 patients in the non tooth mark tongue control group (CON). Fecal specimens were collected from all enrolled patients. Using the Illumina NovaSeq 6000 sequencer, the V3-V4 region of 16S rDNA in total fecal DNA was subjected to high-throughput sequencing, and the intestinal microbiota structure was analyzed with bioinformatics software. Results: The richness and diversity of gut microbiota were significantly reduced in the TMT. The abundance of Lactobacillus in the TMT was significantly lower than that in the CON (P<0.05). The prediction model was constructed based on LEfSe and random forest analysis. It was found that the AUC value was 97.9%. The analysis based on the difference in KEGG pathway enrichment revealed that the level of metabolic pathway function associated with endocrine metabolic and lipid metabolism was decreased in the TMT. Conclusion: The gut microbiota disorder is more significant in NAFLD patients with teeth-marked tongue, characterized by a significant reduction in the richness of the gut microbiota, especially a significant decrease in the abundance of Lactobacillus at the genus level.

Key words：NAFLD; tooth mark tongue; Lactobacillus; gut microbiota

发布时间：2026-01-06

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