苍耳温胆汤对变应性鼻炎大鼠TLR4/MyD88/NF-κB信号通路的影响*

作者：李志军1，景伟超1，王钇杰1，李桃丹2，常钰昕2，王有鹏1

单位：1.黑龙江中医药大学附属第二医院，黑龙江 哈尔滨 150001； 2.黑龙江中医药大学，黑龙江 哈尔滨 150006

引用：引用：李志军，景伟超，王钇杰，李桃丹，常钰昕，王有鹏.苍耳温胆汤对变应性鼻炎大鼠TLR4/MyD88/NF-κB信号通路的影响[J].中医药导报,2025,31(6):36-41.

DOI：10.13862/j.cn43-1446/r.2025.06.007

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摘要：

目的：探讨苍耳温胆汤对变应性鼻炎（AR）大鼠TOLL样受体4（TLR4）/髓样分化因子88（MyD88）/核转录因子-κB/（NF-κB）信号通路的影响。方法：将40只雄性大鼠随机分为空白组、模型组、西替利嗪组和苍耳温胆汤组，每组10只。除空白组外，其余3组大鼠采用含有卵蛋白（OVA）及氢氧化铝[Al(OH)3]的致敏液致敏，建立过敏性鼻炎大鼠模型。各组予相应药物干预7 d。观察记录大鼠鼻炎症状测定评分；采用苏木素-伊红（HE）染色法观察大鼠鼻黏膜组织学形态变化；采用酶联免疫吸附试验（ELISA）检测大鼠血清免疫球蛋白E（IgE）、白介素-6（IL-6）、白介素-10（IL-10）水平；采用蛋白免疫印迹法（Western blotting）检测鼻黏膜TLR4、MyD88、NF-κB蛋白表达；采用逆转录实时定量聚合酶链式反应（RT-qPCR）检测鼻黏膜TLR4 mRNA、MyD88 mRNA、NF-κB mRNA表达。结果：给药后，模型组大鼠鼻炎症状测定评分高于空白组（P<0.05）；苍耳温胆汤组、西替利嗪组大鼠鼻炎症状测定评分均低于模型组（P<0.05）；苍耳温胆汤组大鼠鼻炎症状测定评分与西替利嗪组比较，差异无统计学意义（P＞0.05）。HE染色显示，空白组大鼠鼻黏膜组织形态结构完整，形态正常；模型组大鼠鼻黏膜大量炎症细胞浸润，且细胞排列紊乱；苍耳温胆汤组、西替利嗪组大鼠鼻黏膜组织中炎症细胞浸润程度及细胞排列紊乱程度均低于模型组。模型组大鼠血清IgE、IL-6水平高于空白组（P<0.05），IL-10水平低于空白组（P＜0.05）；苍耳温胆汤组、西替利嗪组大鼠血清IgE、IL-6水平均低于模型组（P＜0.05），IL-10水平均高于模型组（P＜0.05）；苍耳温胆汤组大鼠血清IgE、IL-6水平低于西替利嗪组（P<0.05），IL-10水平高于西替利嗪组（P＜0.05）。模型组大鼠鼻黏膜TLR4、MyD88、NF-κB蛋白相对表达量及TLR4 mRNA、MyD88 mRNA、NF-κB mRNA相对表达量均高于空白组（P＜0.05）；苍耳温胆汤组、西替利嗪组大鼠鼻黏膜TLR4、MyD88、NF-κB蛋白相对表达量及TLR4 mRNA、MyD88 mRNA、NF-κB mRNA相对表达量均低于模型组（P＜0.05）；苍耳温胆汤组大鼠鼻黏膜TLR4、MyD88、NF-κB蛋白相对表达量及TLR4 mRNA、MyD88 mRNA、NF-κB mRNA相对表达量均低于西替利嗪组（P<0.05）。结论：苍耳温胆汤能改善AR大鼠症状及炎症反应，其作用机制可能与调控TLR4/MyD88/NF-κB信号通路有关。

关键词：变应性鼻炎；苍耳温胆汤；分消走泄法；TLR4/MyD88/NF-κB信号通路；大鼠

Abstract：

Objective: To investigate the effect of Cang'er Wendan Tang (CEWDT) on the TOLL-like receptor4 (TLR4)/myeloid differentiation factor 88 (MyD88)/nuclear transcription factor-κB (NF-κB) pathway in allergic rhinitis (AR) rats. Methods: Totall 40 male rats were randomly divided into blank group, model group, cetirizine group and CEWDT group, with 10 rats in each group. Except for the blank group, the rats in the other three groups were sensitised with sensitising solution containing ovalbumin (OVA) and Al(OH)3 to establish an allergic rhinitis rat model. Each group was given corresponding medication intervention for 7 days. The rats' rhinitis symptom scores were observed and recorded. Histological and morphological changes in the nasal mucosa of rats were observed by hematoxylin-eosin (HE) staining. Serum IgE, interleukin-6 (IL-6) and IL-10 levels were detected by enzyme-linked immunosorbent assay (ELISA). Nasal mucosal TLR4, MyD88 and NF-κB protein expression levels were detected by protein Western blotting, and TLR4 mRNA, MyD88 mRNA and NF-κB mRNA expression levels were detected by RT-qPCR. Results: After administration, the rats in the model group showed higher rhinitis symptom scores than those in the blank group (P<0.05). The rats in the CEWDT group and the cetirizine group showed lower rhinitis symptom scores than those in the model group (P<0.05). The difference in  rhinitis symptom scores between CEWDT group and cetirizine group was not statistical (P＞0.05). HE staining showed that the nasal mucosa tissue of rats in the blank group was structurally intact and normal. The nasal mucosa of rats in the model group was infiltrated by a large number of symptomatic cells and the arrangement of cells was disorganized. The degree of inflammatory cell infiltration and disorganization of cells in the nasal mucosa tissue of rats in the CEWDT group and the cetirizine group was lower than that of the model group. The model group showed higher serum IgE and IL-6 levels than blank group (P<0.05), while lower expression level of IL-10 than blank group (P<0.05). The CEWDT group and cetirizine group showed lower serum IgE and IL-6 levels than model group (P<0.05), while higher level of IL-10 than model group (P<0.05). The CEWDT group showed lower serum IgE and IL-6 levels than cetirizine group (P<0.05), while higher serum IL-10 level than cetirizine group (P<0.05). The model group showed higher relative expression of TLR4, MyD88 and NF-κB proteins and relative expression of TLR4 mRNA, MyD88 mRNA and NF-κB mRNA than blank group (P<0.05). The CEWDT group and cetirizine group showed lower relative expression of TLR4, MyD88 and NF-κB proteins and relative expression of TLR4 mRNA, MyD88 mRNA and NF-κB mRNA than model group (P<0.05). The CEWDT group showed lower relative expression of TLR4, MyD88 and NF-κB proteins and relative expression of TLR4 mRNA, MyD88 mRNA and NF-κB mRNA than cetirizine group (P<0.05). Conclusion: Cang'er Wendan Tang can improve clinical symptoms and inflammatory responses in AR rats, and its mechanism of action may be related to regulatory of TLR4/MyD88/NF-κB signalling pathway.

Key words：allergic rhinitis; Cang'er Wendan Tang; dispersing and discharging method; TOLL-like receptor4/myeloid differentiation factor 88/nuclear transcription factor-κB pathway; rat

发布时间：2026-01-03

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