基于脂质过氧化途径探讨黄芪甲苷对高糖诱导人足细胞铁死亡的保护作用*

作者：孙亚利，王彬蒙，丁新月，邢丽娜

单位：上海中医药大学附属普陀医院，上海 200060

引用：引用：孙亚利，王彬蒙，丁新月，邢丽娜.基于脂质过氧化途径探讨黄芪甲苷对高糖诱导人足细胞铁死亡的保护作用[J].中医药导报,2025,31(6):26-30.

DOI：10.13862/j.cn43-1446/r.2025.06.005

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摘要：目的：探讨黄芪甲苷（AS-Ⅳ）通过减少脂质过氧化的发生，对高糖诱导人足细胞（HPC）铁死亡的保护作用。方法：将细胞分为对照组（5.5 mmol/L D-葡萄糖）、模型组（30.0 mmol/L D-葡萄糖）、低剂量给药组（30.0 mmol/L D-葡萄糖＋10.0 μmol/L AS-Ⅳ）和高剂量给药组（30.0 mmol/L D-葡萄糖＋40.0 μmol/L AS-Ⅳ），通过CCK-8计算细胞存活率，Western blotting检测cleaved Caspase-3、PTGS2和GPX4蛋白水平，Hoechst染色后观察细胞凋亡情况，检测细胞MDA、SOD、CAT、GSH-Px水平。结果：与模型组比较，低、高剂量给药组细胞存活率升高（P<0.05），高剂量给药组cleaved Caspase-3表达水平降低（P<0.05），Hoechst染色后显示高剂量给药组细胞凋亡率降低（P<0.01）。低、高剂量给药组MDA水平均降低（P<0.01），SOD水平均升高（P<0.01）；低、高剂量给药组CAT和GSH-Px水平升高（P<0.05或P<0.01）。低、高剂量给药组PTGS2表达水平降低（P<0.01），高剂量给药组GPX4蛋白表达水平升高（P<0.01）。结论：黄芪甲苷可能通过抑制脂质过氧化的发生，减轻高糖诱导的肾小球足细胞铁死亡情况。

关键词：足细胞；糖尿病肾脏病；铁死亡；黄芪甲苷；脂质过氧化

Abstract：

Objective: To investigate the protective effect of astragaloside Ⅳ on high glucose-induced ferroptosis in human podocytes (HPC) by reducing the occurrence of lipid peroxidation. Methods: The cells were divided into control group (5.5 mmol/L D-glucose), model group (30.0 mmol/L D-glucose), low dose group (30.0 mmol/L D-glucose+10.0 μmol/L AS-Ⅳ) and high dose group (30.0 mmol/L D-glucose+40.0 μmol/L AS-Ⅳ). The cell viability was calculated by CCK-8. The protein levels of cleaved Caspase-3, PTGS2 and GPX4 were detected by Western blotting. The apoptosis was observed by Hoechst staining, and the levels of MDA, SOD, CAT and GSH-Px were detected. Results: Compared with the model group, the cell survival rate increased in low dose group and high dose group (P<0.05), and the expression level of cleaved Caspase-3 decreased in high dose group (P<0.05). Hoechst staining showed that the apoptosis rate decreased in high dose group (P<0.01). The levels of MDA decreased in low dose group and high dose group (P<0.01), and the levels of SOD increased in low dose group and high dose group (P<0.01). The levels of CAT and GSH-px in increased in the  low dose group and high dose group (P<0.05 or P<0.01). The expression level of PTGS2 decreased in low dose group and high dose group (P<0.01), while the expression level of GPX4 protein increased in high dose group (P<0.01). Conclusion: Astragaloside Ⅳ may reduce the ferroptosis of glomerular podocytes induced by high glucose according to inhibiting the occurrence of lipid peroxidation.

Key words：podocyte; diabetes kidney disease; ferroptosis; astragaloside Ⅳ; lipid peroxidation

发布时间：2026-01-03

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