基于pERK1/2信号通路探究补肾活血方对狼疮肾炎大鼠肾纤维化的改善作用及机制

作者：沈 瑜1，赵 鑫2，徐三彬1，黄美琴1，麻志恒1

单位：1.上海健康医学院附属崇明医院/上海新华医院崇明分院，上海 201318； 2.上海市浦东新区合庆社区卫生服务中心，上海 201201

引用：引用：沈瑜，赵鑫，徐三彬，黄美琴，麻志恒.基于pERK1/2信号通路探究补肾活血方对狼疮肾炎大鼠肾纤维化的改善作用及机制[J].中医药导报,2025,31(6):21-25.

DOI：10.13862/j.cn43-1446/r.2025.06.004

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摘要：

目的：探究补肾活血方对狼疮肾炎大鼠肾纤维化的改善作用和机制，分析补肾活血方对狼疮肾炎的临床治疗价值。方法：选取40只SD大鼠，通过腹腔注射降植烷来造狼疮肺炎大鼠模型。将大鼠分为4组，即空白组、模型组、泼尼松组和补肾活血方组。对泼尼松组和补肾活血方组用药治疗28 d后，采用酶联免疫吸附试验检测各组大鼠血清中肾功能指标血尿素氮（BUN）和血肌酐（S-Cr）表达量以及炎症因子肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）的含量。对各组大鼠肾组织进行苏木素-伊红（HE）和马松（Masson）染色，进行病理学观察。采用蛋白质印迹法检测蛋白表达水平。结果：与空白组相比，模型组血清肾功能指标BUN和S-Cr水平升高，促炎因子TNF-α、IL-6水平升高，肾组织纤维化程度加重，纤维化面积增加，纤维化过程中的关键分子蛋白转化生长因子β1（TGF-β1）、Ⅰ型和Ⅲ型胶原蛋白表达水平升高。与模型组比较，补肾活血组和泼尼松组的血清肾功能指标BUN和S-Cr水平降低，促炎因子TNF-α、IL-6水平降低，肾纤维化程度减轻，纤维化过程中的关键分子蛋白TGF-β1、Ⅰ型和Ⅲ型胶原蛋白表达水平降低，ERK通路关键蛋白pERK1/2也降低。结论：补肾活血方可能通过抑制pERK1/2通路改善狼疮大鼠肾炎的肾纤维化。

关键词：系统性红斑狼疮；狼疮肾炎；肾纤维化；补肾活血方；pERK1/2通路；大鼠

Abstract：Objective: To explore the effect and mechanism of Bushen Huoxue formula on improving renal fibrosis in lupus nephritis rats, and to analyze the clinical therapeutic value of Bushen Huoxue formula on lupus nephritis. Methods: Totally 40 SD rats were selected to create lupus nephritis rat model by intraperitoneal injection of norphytane. The rats were divided into 4 groups, including blank group, model group, prednisone group and Bushen Huoxue formula group. After 28 days of treatment in prednisone group and Bushen Huoxue formula group, the expression levels of blood urea nitrogen (BUN) and serum creatinine (S-Cr) in serum renal function indexes and the contents of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were detected by enzyme-linked immunosorbent assay (ELISA). Hematoxylin-eosin (HE) and Masson staining were used to observe the pathology of renal tissues in each group. The protein expression level was detected by Western blotting. Result: Compared with the blank group, the serum renal function indexes (BUN and S-Cr) levels, and the pro-inflammatory factor (TNF-α and IL-6) levels were increased in the model group. The degree of renal fibrosis was aggravated, and the fibrosis area was increased in model group. The expression levels of TGF-β1, type Ⅰ and type Ⅲ collagen, the key molecular proteins in the fibrosis process, were increased in model group. Compared with model group, the levels of BUN and S-Cr in serum renal function indexes, and the levels of pro-inflammatory factors TNF-α and IL-6 were decreased in Bushen Huoxue formula group and prednisone group. The degree of renal fibrosis was alleviated Bushen Huoxue formula group and prednisone group. The expression levels of TGF-β1, type Ⅰ and type Ⅲ collagen, the key molecular proteins in the fibrosis process, were decreased in Bushen Huoxue formula group and prednisone group. p-ERK1/2, a key protein in the ERK pathway, was also reducedin Bushen Huoxue formula group and prednisone group. Conclusion: Bushen Huoxue formula may improve renal fibrosis in lupus rat nephritis by inhibiting p-ERK1/2 pathway.

Key words：systemic lupus erythematosus; lupus nephritis; renal fibrosis; Bushen Huoxue formula; p-ERK1/2 signaling pathway; rat

发布时间：2026-01-03

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