血府逐瘀胶囊及其拆方抑制棕榈酸钠诱导的平滑肌细胞泡沫化、线粒体损伤及凋亡*

作者：程 序1，魏 琼1，赵梦竹1,2，刘梦华1,3，张冬梅1

单位：1.北京中医药大学东直门医院，北京 100007； 2.海军军医大学，上海 200433；3.开封市中医院，河南 开封 475000

引用：引用：程序，魏琼，赵梦竹，刘梦华，张冬梅.血府逐瘀胶囊及其拆方抑制棕榈酸钠诱导的平滑肌细胞泡沫化、线粒体损伤及凋亡[J].中医药导报,2025,31(3):45-50.

DOI：10.13862/j.cn43-1446/r.2025.03.008

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摘要：

目的：通过体外实验探讨血府逐瘀胶囊及其拆方对平滑肌源性泡沫细胞的形成及凋亡的影响。方法：将血府逐瘀胶囊拆分为活血方（红花、炒桃仁、牛膝、桔梗、生地黄、甘草）和理气方（枳壳、川芎、柴胡、当归、赤芍、炒桃仁）；制备活血方、理气方及二者混合方的冻干粉；棕榈酸钠诱导小鼠平滑肌细胞（Movas）泡沫化及凋亡并给予药物干预；CCK-8法检测药物对平滑肌细胞活力的影响；油红O染色检测细胞内脂质吞噬及泡沫化；Annexin V-PI检测细胞凋亡情况；ROS检测细胞活性氧释放情况；JC-1检测细胞线粒体膜电位。结果：随着棕榈酸钠诱导浓度的升高，Movas的脂质吞噬情况、相对泡沫化程度和凋亡率逐步升高；500 μmol/L及1 mmol/L棕榈酸钠诱导Movas凋亡率高于60%（P<0.01）。1.6 mg/mL混合方及800 μg/mL理气方能明显抑制Movas泡沫化。800 μg/mL活血方及理气方能显著增加Movas存活率（P<0.01）、抑制Movas早期凋亡率（P<0.01）、抑制Movas活性氧释放（P<0.05），并显著提高线粒体膜电位（P<0.05）。结论：血府逐瘀胶囊及其拆方可抑制棕榈酸钠诱导的平滑肌细胞泡沫化及凋亡，这可能与抑制线粒体的激活从而抑制活性物质ROS的释放有关。

关键词：平滑肌细胞；泡沫细胞；凋亡；线粒体损伤；血府逐瘀胶囊；活血；理气

Abstract：

Objective: To investigate the effect of Xuefu Zhuyu capsule and its disassembly prescription on the formation and apoptosis of smooth muscle derived foam cells by in vitro experiment. Methods: Xuefu Zhuyu capsule was divided into Huoxue (活血) formula[ Honghua (safflower), Chaotaoren (fried peach kernel), Niuxi (achyranthus), Jiegeng (platycodon platycodon), Shengdihuang (rehmannia), and Gancao (licorice)] and Liqi (理气)  formula [Zhiqiao (fructus aurantii), Chuanxiong (chuanxiong), Chiahu (bupleurum), Dangui (angelica sinensis), Chishao (red peony) and Chaotaoren (fried peach kernel)]. The freeze-dried powder of Huoxue formula, Liqi formula and their mixture was prepared. Sodium palmitate was used to induce the foaming and apoptosis of mouse smooth muscle cells (Movas), treating with drug intervention. CCK-8 was used to detect the effect of drugs on Movas activity. Lipid phagocytosis and foaming were detected by oil red O staining. Annexin V-PI was used to detect apoptosis. ROS was used to detect the release of reactive oxygen species. Cell mitochondrial membrane potential was detected by JC-1. Results: With the increase of sodium palmitate induction concentration, the lipid phagocytosis, relative foaming degree and apoptosis rate of Movas increased gradually. The apoptosis rate of Movas induced by 500 μmol/L and 1 mmol/L sodium palmitate reached over 60% (P<0.01). Mixed formula (1.6 mg/mL) and Liqi formula (800 μg/mL) inhibited Movas foaming significantly. Huoxue formula and Liqi formula increased the survival rate of Movas at 800 μg/mL (P<0.01), inhibited the early apoptosis rate of Movas (P<0.01), inhibited the release of Movas ROS (P<0.05), and increased the mitochondrial membrane potential (P<0.05) significantly. Conclusion: Xuefu Zhuyu capsule and its disassembly can inhibit the foam and apoptosis of smooth muscle cells induced by sodium palmitate, which may be related to the inhibition of the activation of mitochondria and the release of ROS.

Key words：smooth muscle cells; foam cells; apoptosis; mitochondrial damage; Xuefu Zhuyu capsule; Huoxue; Liqi

发布时间：2025-12-14

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