基于UPLC-Q-TOF-MS法分析何首乌不同炮制品化学成分及其抗衰老作用*

作者：何秀娟1，王 璇1，黄 蓉1，彭若威1，李晓欣1，刘永刚1，谭 鹏1，2

单位：1.北京中医药大学中药学院，北京 102488； 2.北京市科委中药生产过程控制与质量评价北京市重点实验室，北京 102488

引用：引用：何秀娟，王璇，黄蓉，彭若威，李晓欣，刘永刚，谭鹏.基于UPLC-Q-TOF-MS法分析何首乌不同炮制品化学成分及其抗衰老作用[J].中医药导报,2025,31(2):35-40.

DOI：10.13862/j.cn43-1446/r.2025.02.007

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摘要：

目的：分析何首乌生品、《北京市中药饮片炮制规范》品（北京炮规品）、《中华人民共和国药典》品（《中国药典》品）之间的成分差异及其抗氧化、抗衰老作用。方法：基于UPLC-Q-TOF-MS法对何首乌不同炮制品成分进行分析；测定1，1-二苯基-2-苦肼基自由基（DPPH）清除率，评价何首乌不同炮制品体外抗氧化能力；观察秀丽隐杆线虫脂褐素水平，分析何首乌不同炮制品的抗衰老作用。结果：鉴定出的何首乌不同炮制品成分共30种，3种炮制品共有成分20种。生品、《中国药典》品、北京炮规品的半数清除率分别为0.006 9、0.043 6、0.033 0 mg/mL。生品组、北京炮规品组、《中国药典》品组秀丽隐杆线虫体内的荧光强度均低于空白组（P＜0.001）。结论：何首乌不同炮制品均具有良好的抗氧化和抗衰老能力，且北京炮规品优于《中国药典》品。

关键词：何首乌；秀丽隐杆线虫；超高效液相色谱-串联四极杆飞行时间高分辨质谱；抗氧化；抗衰老

Abstract：

Objective: The composition differences and anti-oxidation and anti-aging effects of Heshouwu (Polygonum multiflorum) raw product, Beijing Traditional Chinese Medicine Preparation Standard processed product(Beijing processed product) and Pharmacopoeia of the People's Republic of China processed product(China Pharmacopoeia processed product) were analyzed. Methods: Based on the ultra-high performance liquid chromatography-tandem quadrupole time-of-flight high resolution mass spectrometry (UPLC-Q-TOF-MS) method, the components of different prepared products of Heshouwu (Polygonum multiflorum) were analyzed. DPPH free radical scavenging rate was determined to evaluate the antioxidant capacity of different prepared Heshouwu (Polygonum multiflorum) in vitro. The lipofuscin level of C. elegans was observed to analyze the effects of different products of Heshouwu (Polygonum multiflorum) on anti-aging. Results: A total of 30 different components were identified from different Heshouwu (Polygonum multiflorum) product, and 20 components were found in 3 different product. The half clearance rate of raw product, China Pharmacopoeia processed product and Beijing processed product were 0.006 9, 0.043 6 and 0.033 0 mg/mL respectively. The raw product group, Beijing processed product group and China Pharmacopoeia processed product group showed lower fluorescence intensity of caenorhabditiselegans than blank group (P<0.001). Conclusion: The different products of Heshouwu (Polygonum multiflorum) have good antioxidant and anti-aging ability, and the Beijing processed product is superior to the China Pharmacopoeia processed product.

Key words：Heshouwu (Polygonum multiflorum); C. elegans; ultra-high performance liquid chromatography-tandem quadrupole time-of-flight high resolution mass spectrometry; anti-oxidation; anti-aging

发布时间：2025-12-07

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