痰瘀互结型动脉粥样硬化大鼠病证结合模型的建立与评估*

作者：孙朝辉1，李雁丹2，李 钦3，解海雪1，魏淑芳1

单位：1.云南中医药大学第一附属医院，云南 昆明 650021； 2.云南省中医中药研究院，云南 昆明 650021； 3.云南中医药大学，云南 昆明 650500

引用：引用：孙朝辉，李雁丹，李钦，解海雪，魏淑芳.痰瘀互结型动脉粥样硬化大鼠病证结合模型的建立与评估[J].中医药导报,2025,31(2):30-34,40.

DOI：10.13862/j.cn43-1446/r.2025.02.006

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摘要：

目的：探究痰瘀互结型动脉粥样硬化大鼠模型的构建方法，考察其可行性并对该模型进行评价，为进一步实验研究奠定基础。方法：选择SD大鼠，采用高脂饲料喂养+一次性腹腔注射维生素D3（60万IU/kg）构建痰瘀互结型动脉粥样硬化大鼠模型，并通过证候评价、病理学观察及生化指标检测，对痰瘀互结型动脉粥样硬化大鼠模型的建立进行评价。结果：模型组大鼠实验后证候总积分高于实验前（P＜0.05），且模型组大鼠实验后证候总积分高于空白组（P＜0.05）；模型组大鼠血清甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）、纤维蛋白原（FIB）、血小板聚集率、血浆黏度、全血黏度（3/s、30/s、50/s、100/s、180/s）、红细胞刚性指数及聚集指数均高于空白组；模型组大鼠高密度脂蛋白胆固醇（HDL-C）、凝血酶原时间（PT）、国际标准化比值（INR）、活化部分凝血活酶时间（APTT）及凝血酶时间（TT）均低于空白组（P＜0.05）。模型组大鼠胸主动脉中层厚度高于空白组（P＜0.05）。HE染色和油红O染色显示模型组大鼠有明显动脉粥样硬化的病理改变。结论：该痰瘀互结型动脉粥样硬化病证结合模型符合痰瘀互结证候的变化特点及动脉硬化病理、生理表现特点，与人类动脉硬化的表现特点具有相似性，造模方法可行。

关键词：动脉粥样硬化；痰瘀互结证；病证结合模型；大鼠

Abstract：

Objective: To explore the construction method of a rat model of atherosclerosis with phlegm-blood stasis, assess its feasibility, and provide an initial evaluation of the model, laying the groundwork for further experimental research. Methods: SD rats were selected and a model of phlegm-blood stasis intertwined atherosclerosis was established by feeding them with a high-fat diet and injecting of vitamin D3 (600 000 IU/kg) in a single dose. The establishment of the sphlegm-blood stasis intertwined atherosclerosis rat model was initially evaluated through symptom evaluation, pathological observation, and biochemical index detection. Results: In the model group rats, the total score of syndrome after the experiment was higher than before (P<0.05), and the model group showed higher total score of syndrome than blank group after the experiment (P<0.05). The model group showed higher TG, TC, LDL-C, FIB, platelet aggregation rate, plasma viscosity, whole blood viscosity (3/s, 30/s, 50/s, 100/s, 180/s), red blood cell rigidity index, and aggregation index than blank group, while lower HDL-C, PT, INR, APTT, and TT than blank group (P<0.05). The model group showed higher thickness of the middle layer of the thoracic aorta than that blank group (P<0.05). According to HE staining and oil red O staining in pathological analysis, the model group showed obvious atherosclerotic pathological changes. Conclusion: This phlegm-blood stasis type atherosclerosis syndrome model combining Chinese medicine syndrome and Western medicine pathology meets the changes in the phlegm-blood stasis syndrome and the pathological and physiological characteristics of atherosclerosis, and it is similar to the expression characteristics of human atherosclerosis. The modeling method is feasible.

Key words：atherosclerosis; phlegm-blood stasis syndrome; model of combination of disease and syndrome; rat

发布时间：2025-12-07

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