健脾益肾方调控Notch通路对5-氟尿嘧啶诱导的骨髓功能抑制的影响*

作者：袁结文，邹铭斐，刘丽丽

单位：佛山市第二人民医院，广东 佛山 528000

引用：引用：袁结文，邹铭斐，刘丽丽.健脾益肾方调控Notch通路对5-氟尿嘧啶诱导的骨髓功能抑制的影响[J].中医药导报,2025,31(2):19-23.

DOI：10.13862/j.cn43-1446/r.2025.02.004

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摘要：

目的：探究健脾益肾方对5-氟尿嘧啶诱导的骨髓功能抑制的影响。方法：将60只BALB/c小鼠随机分为6组（每组10只）：空白对照组、化疗组、阳性对照组、中药低剂量组、中药中剂量组、中药高剂量组。通过血细胞分析仪检测各组小鼠血液样本中红细胞、白细胞和血小板数量及血红蛋白水平。通过酶联免疫吸附试验（ELISA）分析各组小鼠血清中造血因子[白介素-3（IL-3）、IL-6、促红细胞生成素（EPO）]的水平。通过流式细胞术分析各组小鼠骨髓细胞的凋亡率。通过蛋白免疫印迹分析各组小鼠骨髓细胞Notch通路相关蛋白表达水平。结果：与空白对照组比较，化疗组的小鼠体质量增长率明显降低（P＜0.05），化疗组的小鼠血细胞数量及血红蛋白水平明显降低（P＜0.05），造血因子水平明显降低（P＜0.05），骨髓组织受到严重破坏，细胞凋亡率明显上升（P＜0.05），Notch1、Notch2及Notch3蛋白表达水平下降（P＜0.05）。与化疗组比较，中药各剂量组及阳性对照组小鼠体质量增长率明显升高（P＜0.05），血细胞数量及血红蛋白水平明显增加（P＜0.05），造血因子水平明显升高（P＜0.05），骨髓组织破坏得到改善，细胞凋亡率明显降低（P＜0.05），Notch1、Notch2及Notch3蛋白表达水平升高（P＜0.05）。结论：健脾益肾方对5-氟尿嘧啶诱导的骨髓功能抑制具有改善作用，并且对骨髓功能的改善作用与激活Notch信号通路密切相关。

关键词：健脾益肾方；5-氟尿嘧啶；骨髓功能；化疗；小鼠

Abstract：

Objective: To investigate the effect of Jianpi Yishen Formula on bone marrow function in mice after fluorouracil chemotherapy. Methods: Mice were randomly divided into 6 groups (10 mice in each group), including blank control group, chemotherapy group, positive control group, low-dose Chinese medicine group, medium-dose Chinese medicine group and high-dose Chinese medicine group. The number of red blood cells, white blood cells, platelets and hemoglobin in blood samples of mice was detected by hematology analyzer in each group. The levels of hematopoietic factors (IL-3, IL-6 and EPO) in the serum of mice were analyzed by ELISA in each group. The apoptosis rate of bone marrow cells was analyzed by flow cytometry in each group. The expression levels of Notch pathway-related proteins in bone marrow cells of each group were analyzed by Western blotting. Results: Compared with the blank control group, the growth rate of mouse body mass was significantly reduced in chemotherapy group (P<0.05). The number of blood cells and hemoglobin level significantly decreased in chemotherapy group (P<0.05), and the levels of hematopoietic factors significantly reduced in chemotherapy group (P<0.05). The bone marrow tissue was severely damaged, and the apoptosis rate was significantly increased in chemotherapy group (P<0.05). The expressions of Notch1, Notch2 and Notch3 proteins were decreased in chemotherapy group (P<0.05). Compared with the chemotherapy group, the growth rate of mouse body mass was significantly increased in Chinese medicine groups and the positive control group (P＜0.05). The number of blood cells and hemoglobin level significantly increased in Chinese medicine groups and positive control group (P<0.05), and the levels of hematopoietic factors significantly increased in Chinese medicine groups and positive control group (P<0.05). The bone marrow tissue was improved, and the apoptosis rate was significantly decreased in Chinese medicine groups and positive control group (P<0.05). The expressions of Notch1, Notch2 and Notch3 proteins were increased in Chinese medicine groups and positive control group (P<0.05). Conclusion: Jianpi Yishen Formula can improve bone marrow function in mice after fluorouracil chemotherapy, and the effect is closely related to the activation of Notch signaling pathway.

Key words：Jianpi Yishen Formula; fluorouracil; bone marrow function; chemotherapy; mice

发布时间：2025-12-07

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